Surface Plasmon Resonance (SPR) is an advanced analytical technique used to study molecular interactions in real time. SPR is a label-free technique that enables the investigation of interactions between molecules at the nanoscale. It relies on the phenomenon of localized surface plasmon resonance (LSPR) to detect changes in refractive index, making it suitable for studying binding events, such as protein-protein interactions or molecular recognition, in a wide range of fields.
When combined with gold nanoparticles (AuNPs), SPR becomes a powerful tool with enhanced capabilities. AuNPs are used in conjunction with SPR to improve the sensitivity, selectivity, and versatility of the technique. They amplify the SPR signal, making it easier to detect low-concentration analytes and enabling multiplexed analysis. Additionally, AuNPs can be functionalized with specific molecules, enhancing their ability to interact with target analytes.
Enhanced Sensitivity: The integration of AuNPs amplifies the sensitivity of SPR instruments. This enhancement allows for the detection of even the minutest changes in refractive index, making it possible to investigate low-concentration analytes with remarkable precision. Our Gold NanoUrchins further extend this property, providing unique SPR shift differences for greater sensitivity and resolution.
Multiplexing Capability: AuNPs introduce multiplexing to SPR, a key advantage in studying multiple interactions simultaneously. By attaching distinct ligands to various AuNPs, researchers can investigate numerous molecular binding events in a single experiment. This capability is particularly valuable in the study of complex biological systems, where a multitude of interactions occur concurrently. It streamlines high-throughput screening and accelerates comprehensive data collection.
Selective Functionalization: The versatility of AuNPs allows for precise functionalization with specific recognition elements such as antibodies, aptamers, or DNA probes. This selectivity empowers researchers to tailor AuNPs to interact exclusively with target analytes. The result is a significant increase in the accuracy and specificity of SPR-based assays, particularly in bioanalysis. Researchers can effectively discriminate between different molecules, enhancing the quality of data and insights obtained.
Real-time Kinetic Insights: Integrating AuNPs with SPR instruments facilitates the real-time examination of binding kinetics. By immobilizing one binding partner on the SPR sensor surface and the other on the surface of AuNPs, researchers gain a profound understanding of association and dissociation rates. This approach offers invaluable insights into the thermodynamics and mechanisms governing molecular interactions, providing a comprehensive view of binding dynamics.
Whether you're seeking to delve deeper into this technology, require assistance in developing your assays, or are eager to explore the possibilities it offers, our team is ready to assist. Contact us today to learn more our services and how we can tailor our expertise to your unique requirements.