Enzyme-linked Aptasorbent Assay (ELASA) is a cutting-edge immunoassay that applies the specificity of aptamers for precise detection of target analytes in various sample types. Aptamers, often referred to as "chemical antibodies," are single-stranded oligonucleotides (DNA or RNA) specifically bind to targets by folding into secondary or tertiary structures. Aptamers have unique advantages such as high affinity, stability, and flexibility, positioning ELASA as a powerful tool for research, diagnostics, and biotechnology applications.
Why ELASA?
ELASA represents the forefront of molecular diagnostics and research with applications across diverse fields, including diagnostic tools, biomarker discovery, biotechnology, fundamental research, environmental monitoring, and drug development. The key benefits of ELASA are:
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High Specificity and Affinity: Aptamers used in ELASA exhibit high specificity and affinity for their target molecules, including small molecules. This results in greater accuracy and reduced cross-reactivity.
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Customizability: ELASA is highly customizable. Researchers can select aptamers that specifically bind to their target analyte, tailoring assays to unique requirements.
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Stability: Aptamers are stable under a wide range of conditions, making ELASA robust and adaptable to different sample types and environments.
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Cost-Efficiency: ELASA may reduce costs associated with antibody production and optimization, offering a more budget-friendly alternative.
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Reduced Batch-to-Batch Variability: Unlike antibodies, aptamers can be synthesized consistently, reducing batch-to-batch variability, and ensuring assay reliability.
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Minimal Animal Use: Aptamers are synthesized in vitro, eliminating the need for animal immunization, aligning with ethical considerations.
Different formats of ELASA
Direct ELASA: Usually used for purified target molecules and screening aptamer for target binding, relatively straightforward and quicker assay.
Indirect ELASA: Employed when requiring increased sensitivity, amplifies signals through a secondary antibody with a reporter molecule.
Sandwich ELASA: Used for the target present in low concentrations, it provides high sensitivity and specificity by utilizing two different aptamers or aptamer-antibody pair.
Competitive ELASA: Ideal for small molecule targets, competing with a reference target; presence of target in sample will inhibit binding of detection aptamer to the reference target.
Why Choose Us?
Our CytoGroup team has over 20 years of experience in assay development, sample handling, data analysis, and proteomics. We offer comprehensive ELASA Development Services tailored to your research and assay needs. Our team of experienced scientists specializes in aptamer selection, assay design, optimization, and validation to deliver reliable and reproducible results. Our service has several advantages, including:
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Competitive pricing
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Compliance with our robust quality control
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Experienced R&D and project operations team
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Designing detection development strategies tailored to your research goals
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After-sales support services from a team of experienced scientists
ELASA development service
We develop the most suitable ELASA for you. According to the required sensitivity and technology, we are capable of developing colorimetric analysis and fluorescence analysis. We offer a variety of ELASA development services, including direct, indirect, sandwich, competitive ELASA testing, and more. Our scope of services includes but are not limited to:
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Binding analysis
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Aptamer screening
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Target quantification
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Pre-coated microplates for ELASA
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Intra- and inter-assay precision
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Specificity/cross-reactivity with a range of related targets
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Linearity
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LOQ/LOD analysis
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Bulk manufacturing
Project workflow
At Cytodiagnostics, we are dedicated to advancing your research and diagnostic goals. Our commitment to innovation, accuracy, and quality assurance sets us apart in the field of ELASA development. Unlock the potential of ELASA technology with our expert team by your side. Contact us today to learn more about our ELASA Development Services and explore how we can tailor our expertise to your unique requirements.