A lateral flow assay (LFA) is a simple and cost-effective diagnostic tool that is commonly used for the rapid detection of specific analytes in a sample.
- Sample pad: This is where the sample is applied. It is usually made of nitrocellulose or cellulose and is designed to absorb and distribute the sample evenly across the test strip.
- Conjugate pad: This pad contains the reagents required for the assay, such as antibodies or enzymes, that will interact with the analyte in the sample.
- Nitrocellulose membrane: This layer acts as a filter to separate the analyte from the sample. The membrane is impregnated with capture antibodies that will bind to specific antigens in the sample.
- Test line: This line indicates the presence of the analyte being tested for. It appears as a colored band if the analyte is present in the sample.
- Control line: This line is usually present on the test strip and is used to indicate that the assay is working properly. It is usually a colored band that appears if the test is performed correctly and the reagents are still active.
- Absorbent pad: This pad is located at the end of the test strip and is used to absorb any excess fluid, ensuring that the test results are not compromised.
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Custom Lateral Flow Assay Development
If you are looking to develop your own lateral flow assays but find the process challenging, our assay development branch, CytoGroup, is here to assist. Specializing in the development of biosensors and diagnostic tools, CytoGroup offers comprehensive services to transform your concept into a fully functional commercial product. Our team of experts will guide you through every stage of development, from design to manufacturing, ensuring your assay meets the highest standards of quality and performance. Learn more about how CytoGroup can assist in your lateral flow assay development. |
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